The genetic structure of silver fir in small isolated populations in sub-Mediterranean zone of Bosnia and Herzegovina and Croatia was analysed using biochemical markers. Biochemical markers were used for the analysis: 9 enzyme systems and 16 gene loci, with 11 of them being polymorphic, with 38 alleles in total. The average number of alleles per locus ranged from 1.8125 to 2.0625. Genetic multi loci diversity ranged from 17.8568 to 29.4664, and gene pool diversity ranged from 1.1684 to 1.1958, while actual heterozygosity ranged from 0.1113 to 0.1706. The analysed populations showed inbreeding as indicated by the positive size of the fixation index with low degree of population differentiation and total differentiation (Dj) 0.1468. Based on the obtained results, and also after comparing them with the results from other similar studies, there are indications that small isolated populations of silver fir in the sub-Mediterranean zone have specific genetic structure, although with autochthonous gene fond distinctive for the Western Balkans.
