The use of biostimulants in coffee cultivation stands out as a sustainable option for agricultural efficiency, not only in terms of productivity but also in phytosanitary management. This work was developed in order to evaluate the effect of a freshwater algae extract, based on Chlorella sp. and enriched with Azospirillum sp., on the growth, yield, and beverage quality of Arabica coffee. The experiment was conducted at the IFSULDEMINAS Campus Inconfidentes in a randomized block design with eight treatments and three replications, at different phenological stages. Growth, yield, phytosanitary, and coffee quality variables were evaluated. Data were subjected to analysis of variance (ANOVA), principal component analysis, and a similarity network, in which correlations among data within a set were analyzed, enabling a more illustrative visualization of the relationships among variables. No significant differences were observed among the analyzed parameters, a result that may be explained by the doses used, which opens possibilities for future research. It is concluded that the application of the freshwater algae-based biostimulant at various phenological stages did not influence the growth or yield of Arabica coffee.