Three Tubulin Genes of Trichoderma harzianum: Alpha, Beta and Gamma

Ano: 2010 | Volume: 53 | Número: 4

Autores: Min Li, Qian Yang, Jinzhu Song

Palavras-chave: trichoderma harzianum, tubulin, codon usage bias, motif

Resumo Inglês:

Three tubulin genes of Trichoderma harzianum were cloned followed genomic walking procedure. The tubulins
showed high degree of amino acid homology with other fungal tubulins and were homologous with each other with
32 to 38% amino acid identity. Three measures for the degree of codon usage bias indicated the presence of bias in
all the sequences, suggesting high expression levels in all the genes. Protein structures were modeled to provide the
basis for understanding the tubulin's properties and its interactions with microtubule-associated proteins. Potential
motifs were also postulated.