This work has as objective to determine the genetic divergencebetween local populations of Araucaria angustifolia by isozyme markers.For this, trees in Irati, PR and Caçador, SC were sampled, for each tree sixlocus were evaluated and observed the alleles of endosperm and embryos(homozygous and heterozygous). The results were explored by multivariatetechniques as Analysis of Group where the similarities between the experimentalunits were obtained by the measures Euclidian and Manhattan. Thelinkage methods used to perform the groups were: Ward, NextNeighborhood and Average Linkage. It was observed the smallest geneticdivergence between local population samples of Pristine Forest and LoggedForest in Irati, even as Capão 3 and Capão 4 in Caçador. The Planted Forestin Irati and the local populations of Capão 1 and Capão 2 in Caçador werethe most critical regions observed for conservation.
