Solid State Production of Manganese Peroxidases Using Arecanut Husk as Substrate

Brazilian Archives Of Biology And Technology

Rua Professor Algacyr Munhoz Mader 3775, CIC
Curitiba / PR
Telefone: (41) 3316-3012
ISSN: 15168913
Editor Chefe: Carlos Ricardo Soccol
Início Publicação: 30/11/1946
Periodicidade: Bimestral
Área de Estudo: Biologia geral

Solid State Production of Manganese Peroxidases Using Arecanut Husk as Substrate

Ano: 2010 | Volume: 53 | Número: 3
Autores: Akhila Rajan, Jayalakshmi Gopinatha Kurup, Tholath Emilia Abraham
Autor Correspondente: Tholath Emilia Abraham | [email protected]

Palavras-chave: arecanut husk, solid state fermentation, manganese peroxidase, phanerocheate chrysosporium, phanerocheate sp

Resumos Cadastrados

Resumo Inglês:

The lignocellulosic biomass from arecanut husk (Areca catechu Linnaeus) was evaluated as a new substrate for
cultivation of Phanerochaete chrysosporium and Phanerochaete sp for solid state fermentation of manganese
peroxidase (MnP). Arecanut had a moisture content of 79.84 % for ripe nut husk whereas green nut husk had 68.39
% moisture and a pH of 5.0, 3.0 and 7.0 for raw, ripe and dry husk. Reducing sugar content was 14.31, 19.21 and
1.77(mg/g of husk) for raw, ripe and dry nut husk, respectively. Non reducing sugar was 1.04(mg/g of husk) for raw
and 0.68 (mg/g of husk) for dry husk. Solid state fermentation carried out at different pH showed optimum enzyme
production at pH 6.0 (52.60 IU/g) for P.chrysosporium and pH 5.0 (44.08 IU/g) for Phanerochaete sp. Optimum
temperature was 30 ± 2° C for both the organisms. Lower concentration of MnSO4 (0.1 mM MnSO4) induced
maximum enzyme production in P.chrysosporium whereas Phanerochaete sp. required 1 mM MnSO4 for induction.
Absence of carbon and nitrogen stimulated enzyme production in P.chrysosporium while Phanerochaete sp. needed
nitrogen. Enzyme was partially purified by ammonium sulphate precipitation followed by ion exchange