Solid State Production of Manganese Peroxidases Using Arecanut Husk as Substrate
Brazilian Archives Of Biology And Technology
Solid State Production of Manganese Peroxidases Using Arecanut Husk as Substrate
Autor Correspondente: Tholath Emilia Abraham | [email protected]
Palavras-chave: arecanut husk, solid state fermentation, manganese peroxidase, phanerocheate chrysosporium, phanerocheate sp
Resumos Cadastrados
Resumo Inglês:
The lignocellulosic biomass from arecanut husk (Areca catechu Linnaeus) was evaluated as a new substrate for
cultivation of Phanerochaete chrysosporium and Phanerochaete sp for solid state fermentation of manganese
peroxidase (MnP). Arecanut had a moisture content of 79.84 % for ripe nut husk whereas green nut husk had 68.39
% moisture and a pH of 5.0, 3.0 and 7.0 for raw, ripe and dry husk. Reducing sugar content was 14.31, 19.21 and
1.77(mg/g of husk) for raw, ripe and dry nut husk, respectively. Non reducing sugar was 1.04(mg/g of husk) for raw
and 0.68 (mg/g of husk) for dry husk. Solid state fermentation carried out at different pH showed optimum enzyme
production at pH 6.0 (52.60 IU/g) for P.chrysosporium and pH 5.0 (44.08 IU/g) for Phanerochaete sp. Optimum
temperature was 30 ± 2° C for both the organisms. Lower concentration of MnSO4 (0.1 mM MnSO4) induced
maximum enzyme production in P.chrysosporium whereas Phanerochaete sp. required 1 mM MnSO4 for induction.
Absence of carbon and nitrogen stimulated enzyme production in P.chrysosporium while Phanerochaete sp. needed
nitrogen. Enzyme was partially purified by ammonium sulphate precipitation followed by ion exchange
chromatography.
