Respirometry Kinetics of Phenol Oxidation by Comamonas testosteroni Pb50 under Various Conditions of Nutritional Stress

Ano: 2010 | Volume: 53 | Número: 6

Autores: Jan Paca, Alena Kosteckova, Leona Pacova, Ales Prell, Martin Halecky, Jan Paca Jr, Marie Stiborova, Evguenii Kozliak, Carlos Ricardo Soccol

Palavras-chave: respirometry, kinetics, phenol, nutrition stress

Resumo Inglês:

Kinetics of phenol biodegradation using suspended biomass of Comamonas testosteroni Pb50 (monoculture) was
measured under conditions of nutrient abundance, limitation, and prolonged cell starvation in a fed-batch reactor,
with phenol being the sole carbon and energy source. The pre-washed cells were applied for measurement of the
phenol and oxygen uptake rates at varied starting phenol concentrations with the kinetic parameters calculated
using the Haldane model. The results revealed that nutrient limitation significantly suppressed the maximum value
of exogenous respiration rate while the endogenous respiration rate, affinity and tolerance to phenol increased. By
contrast, cell starvation resulted in a drop of both the exogenous and endogenous respiration rates by an order of
magnitude.