α-Galactosidase was produced by Aspergillus oryzae on red gram plant waste-wheat bran based media in solidstate
fermentation (SSF). Optimum temperature for a-galactosidase production was 35 0C and upto 4 cm of bed
height of substrate had no inhibitory effect on enzyme production. Hydrolysis of galactooligosaccharides in soymilk
was carried out by α-galactosidase. Optimum temperature and pH for the hydrolysis of raffinose and stachyose of
soymilk were 550C and 5.2-6.2, respectively. The enzymatic treatment for 3 h completely removed the raffinose
oligosaccharides in soymilk. Crude extract also showed considerable amount of invertase activity.
