Production of Bio-inseticide Bacillus thuringiensis var. israelensis in Semicontinuous Processes Combined with Batch Processes for Sporulation

Brazilian Archives Of Biology And Technology

Endereço:
Rua Professor Algacyr Munhoz Mader 3775, CIC
Curitiba / PR
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Site: http://www.scielo.br/babt
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ISSN: 15168913
Editor Chefe: Carlos Ricardo Soccol
Início Publicação: 30/11/1946
Periodicidade: Bimestral
Área de Estudo: Biologia geral

Production of Bio-inseticide Bacillus thuringiensis var. israelensis in Semicontinuous Processes Combined with Batch Processes for Sporulation

Ano: 2011 | Volume: 54 | Número: 1
Autores: Millena da Silva, Agenor Furigo Junior, Sandra Aparecida Furlan, Ozair Souza
Autor Correspondente: Ozair Souza | [email protected]

Palavras-chave: semicontinuous fermentation, combined process, bti, bioinsecticide

Resumos Cadastrados

Resumo Inglês:

The aim of this work was to study the production of the bioinsecticide using the aerobic cultivation. Two-stage
cultivation process was used: aerobic vegetative growth and end-phase of sporulation (with aeration and without
aeration). The cuts of working volume evaluated were 20, 40, 60 and 80 %. The highest value of spore
concentration (2.17x109 CFU/mL) and spore productivity (7.2x107 CFU/mL.h) were observed using SP60. The
lethal concentration (CL50) obtained through the aeration process and non-aeration process were 0.21 and 0.05
mg/L, respectively. The specific potential of the bioinsecticide produced without aeration (0.025 mg/10-12 CFU) was
two times higher than that obtained with aeration (0.05 mg/10-12 CFU).