Production and Characterization of Endo-Polygalacturonase from Aspergillus niger in Solid-state Fermentation in Double-Surface Bioreactor

Brazilian Archives Of Biology And Technology

Endereço:
Rua Professor Algacyr Munhoz Mader 3775, CIC
Curitiba / PR
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Site: http://www.scielo.br/babt
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ISSN: 15168913
Editor Chefe: Carlos Ricardo Soccol
Início Publicação: 30/11/1946
Periodicidade: Bimestral
Área de Estudo: Biologia geral

Production and Characterization of Endo-Polygalacturonase from Aspergillus niger in Solid-state Fermentation in Double-Surface Bioreactor

Ano: 2011 | Volume: 54 | Número: 2
Autores: Diogo Henrique Hendges, Queli Montanari, Eloane Malvessi, Mauricio Moura da Silveira
Autor Correspondente: Diogo Henrique Hendges | [email protected]

Palavras-chave: aspergillus niger, double-surface bioreactor, endo-polygalacturonase, solid-state fermentation, enzyme stability

Resumos Cadastrados

Resumo Inglês:

Endo-polygalacturonase (endo-PG) production by Aspergillus niger T0005/007-2 in solid medium with 170 mm of
height was evaluated in a cylindrical double surface bioreactor in 96-h experiments. Cell concentration close to 92
mg.g-1dm (mg per g of dry medium) in the standard condition (static) was achieved, whereas in tests under forced
aeration of 1.4 and 2.8 L.min-1.Kg-1mm (L of air per minute per Kg of moist medium) and with the central shaft
fungal biomass attained approximately 100 mg.g-1dm. Superior endo-PG activity was obtained with the centralshaft
system, 78 U.g-1dm (units per g of dry medium). Forced aeration and pressure pulse showed no positive effect
on the production of endo-PG, 45 U.g-1dm and 28 U.g-1dm, respectively. None of the conditions evaluated was
efficient for medium temperature control. Endo-PG was stable up to 40ºC. The activity decreased in 50% after 120
minutes at 50ºC, which is a temperature normally found during this process.