Physiochemical Parameters Optimization for Enhanced Nisin Production by Lactococcus lactis (MTCC 440)

Ano: 2010 | Volume: 53 | Número: 1

Autores: Puspadhwaja Mall, Bijaya Kumar Mohanty, Dhananjay Bhawanishankar Patankar, Rustom Mody, Rashbehari Tunga

Palavras-chave: nisin, lactococcus lactis, streptococcus agalactae, skimmed milk

Resumo Inglês:

The influence of various physiochemical parameters on the growth of Lactococcus lactis sub sp. lactis MTCC 440
was studied at shake flask level for 20 h. Media optimization (MRS broth) was studied to achieve enhanced growth
of the organism and also nisin production. Bioassay of nisin was done with agar diffusion method using
Streptococcus agalactae NCIM 2401 as indicator strain. MRS broth (6%, w/v) with 0.15μg/ml of nisin supplemented
with 0.5% (v/v) skimmed milk was found to be the best for nisin production as well as for growth of L lactis. The
production of nisin was strongly influenced by the presence of skimmed milk and nisin in MRS broth. The
production of nisin was affected by the physical parameters and maximum nisin production was at 300C while the
optimal temperature for biomass production was 370C.