ISOLATION AND PURIFICATION OF COMPLEX II FROM PROTEUS MIRABILIS STRAIN ATCC 29245
Brazilian Journal Of Microbiology
ISOLATION AND PURIFICATION OF COMPLEX II FROM PROTEUS MIRABILIS STRAIN ATCC 29245
Autor Correspondente: Khadija Shabbiri | [email protected]
Palavras-chave: Proteus mirabilis, complex II, cytochrome b
Resumos Cadastrados
Resumo Inglês:
A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC
29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1)
containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration.
The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with
molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b
per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form
showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The -band was shifted from 557.0 nm to 556.8
nm in pyridine ferrohemochrome spectrum. The succinate: quinone oxidoreductase activity was found to
be high in this microorganism.
