IDENTIFICATION OF GRP75 AS A NOVEL PRES1 BINDING PROTEIN USING A PROTEOMICS STRATEGY

Ano: 2010 | Volume: 41 | Número: 2

Autores: Lunbiao Cui, Yiyue Ge, Yuhua Qi, Zhiyang Shi, Yongjun Jiao, Xian Qi, Xiangjun Zhai, Hua Wang

Palavras-chave: HBV, PreS1, Proteomics, GRP75

Resumo Inglês:

The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus
(HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase
(GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucoseregulated
proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75
were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in
mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their
critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches
our knowledge about molecular mechanism of HBV infection.