Extracellular Enzymes of Fusarium graminearum Isolates

Ano: 2010 | Volume: 53 | Número: 4

Autores: Gisele Eleonora Kikot, Roque Alberto Hours, Teresa María Alconada

Palavras-chave: extracellular enzymes, fusarium graminearum, pectinases, cellulases, hemicellulases

Resumo Inglês:

Fusarium graminearum isolates from three different agroecological regions in Argentina were examined according
to the production of different extracellular enzyme activities of potential biotechnological interest: pectinases
(PGase: polygalacturonase and PMGase: polymethylgalacturonase), cellulase (CMCase: carboxymethylcellulase)
and hemicellulase (xylanase). The isolates were grown in minimum salt medium supplemented with 0.25% glucose,
0.125% citric pectin and 0.125% oat bran as carbon sources and/or enzyme inducers. PGase activity was detected
early (after two days of incubation) in all the cultures; it was found to be the highest for all the isolates. PMGase
was high only for those isolates of the II region. CMCase and endoxylanase activities were particularly found at late
stages (after four and seven days of incubation, respectively) and the maximum values were lower than pectinase
activities.