Effect of Picloram, Additives and Plant Growth Regulators on Somatic Embryogenesis of Phyla nodiflora (L.) Greene

Brazilian Archives Of Biology And Technology

Endereço:
Rua Professor Algacyr Munhoz Mader 3775, CIC
Curitiba / PR
0
Site: http://www.scielo.br/babt
Telefone: (41) 3316-3012
ISSN: 15168913
Editor Chefe: Carlos Ricardo Soccol
Início Publicação: 30/11/1946
Periodicidade: Bimestral
Área de Estudo: Biologia geral

Effect of Picloram, Additives and Plant Growth Regulators on Somatic Embryogenesis of Phyla nodiflora (L.) Greene

Ano: 2011 | Volume: 54 | Número: 1
Autores: Abdul Bakrudeen Ali Ahmed, Adhikarla Suryanarayana Rao, Mandali Venkateswara Rao, Rosna Mat Taha
Autor Correspondente: Abdul Bakrudeen Ali Ahmed | [email protected]

Palavras-chave: somatic embryogenesis (ses), picloram, ascorbic acid, coconut milk, plant growth regulators, murashige and skoog medium

Resumos Cadastrados

Resumo Inglês:

The present study describes the plant regeneration via somatic embryogenesis in suspension culture derived from
the leaf and stem explants of Phyla nodiflora. The medium type, plant growth regulators, complex extract (coconut
milk and malt extract) and anti-oxidant (activated charcoal, ascorbic acid, Polyvinylpyrrolidone and citric acid)
markedly influenced the embryo regeneration of P. nodiflora. MS with 2,4-D and activated charcoal (10 mg/L) gave
the highest stimulation of embryogenic callus growth. Optimized callus was transfered into suspension culture,
which showed the globular, heart shaped embryos in MS with 2,4-D + BA + picloram (0.1 mg/L), coconut milk (10
ml/L), citric acid (100 mg/L) on 6th subcultures. Further development stages such as torpedo and cotyledonary
stage embryos and fostered maturation of embryos were observed at 8th and 10th subculture. However, the high
frequency embryo germination and plantlet (45 plants/20 mg cotyledonary stages embryos) formation was obtained
in half-strength MS medium without growth regulators from cotyledonary embryos. All the plantlets established in
the field exhibited morphological characters similar to those of the mother plant.