Effect of Different Forms of Prostaglandin F2α Analogues Administration on Hormonal Profile, Prostaglandin F2α Binding Rate and Reproductive Traits in Akkaraman Sheep During the Breeding Season

Ano: 2010 | Volume: 38 | Número: 4

Autores: Ali Risvanli, Orkun Demiral, Murat Abay, Nevzat Saat, Tayfur Bekyurek, Fatih Kulahci, Secil Niksaroglu, Tansel Ansal Balci

Palavras-chave: prostaglandin f2?, analogue, anti-prostaglandin f2?

Resumo Inglês:

Background: We also aimed to detect endogenous anti-prostaglandin F2α antibodies and the relationship between
these antibodies and fertility parameters such as pregnancy rates after the use of PGF2α. Synchronization of estrus
with the PGF2αis sometimes ineffective in sheep. This situation is to bring about due to the lack of a functional
corpus luteum, given before day 5-6 of the estrous cycle. But, it can be suggested that the immunity developing for
endogenous or exogenous PGF2α analogues or metabolites.
Materials, Methods & Results: The efficacy of single dose prostaglandin F2α analogues such as dinoprost tromethamine,
cloprostenol sodium and d-cloprostenol was studied in 60 iparous and multiparous Akkaraman sheep. The animals
were grouped in three groups (n = 20). Each group was randomly divided into two subgroups comprising a minimum
of 10 iparous and multiparous animals. All individual serum samples collected from one flock in Kayseri province
of middle Anatolian Turkey, in the period from August 2009 to October 2009. The Animals were kept in free stall and
indoors at night, and had access to grazing outdoors most of the day. Prostaglandin F2α was administered to the
animals one week after the first estrus and blood samples were collected three times (at 0, 24th and 48th h after the
injection). 10 mL blood was collected respectively via jugular venipuncture. The estrus cycle of each ewe was
followed 4 times at a 6-h interval (to determine differences of estrus time between the groups) using teaser rams. The
concentrations of progesterone and estradiol in the samples were measured using the commercial Enzyme-Linked
Immunosorbent Assay (ELISA) kits. Furthermore, the presence of anti-prostaglandin F2αantibodies in these samples
was detected by scintillation counter using radioactively-labeled [5,6,8,9,11,12,14,15(n)-3H] PGF2α. There was a
significant decrease in the progesterone levels in both iparous and multiparous animals, to which cloprostenol
sodium was administered on the day of administration and after 24 and 48h, compared to other groups. There was no
significant difference between the groups in terms of pregnancy rates. Anti-prostaglandin F2α antibodies were detected
in all blood samples, although the levels were low. The binding rates for PGF2α in sera collected at the 48th hour after
a single dose d-cloprostenol administration were lower than those on the day of injection in multiparous animals.
However, it was considered that these antibodies did not have a sufficient level to affect parameters such as pregnancy
rates in Akkaraman sheep.
Discussion: However, it was found that the effects of three PGF2α analogues were not different in terms of
pregnancy rates. It was also concluded in this study that anti-prostaglandin F2α antibodies did not show any effect on
parameters such as pregnancy rates in Akkaraman sheep. In addition, it was concluded that it is required to perform
further studies with more details related to the development of anti-prostaglandin F2α antibodies and their effect
mechanism on fertility in Akkaraman sheep.