DIFFERENTIATION OF BOVINE CORONAVIRUS (BCoV) GENOTYPES BY A RESTRICTION ENZYME ASSAY

Brazilian Journal Of Microbiology

Endereço:
Av. Prof. Lineu Prestes, 2415 Cidade Universitária
São Paulo / SP
Site: http://www.scielo.br/scielo.php?script=sci_serial&pid=1517-8382
Telefone: (11) 3813 9647
ISSN: 15178382
Editor Chefe: Adalberto Pessoa Junior
Início Publicação: 31/12/1970
Periodicidade: Trimestral
Área de Estudo: Microbiologia

DIFFERENTIATION OF BOVINE CORONAVIRUS (BCoV) GENOTYPES BY A RESTRICTION ENZYME ASSAY

Ano: 2010 | Volume: 41 | Número: 3
Autores: Sibele Pinheiro de Souza, Karen Miyuki Asano, Thaisa Lucas Sandri, Iracema Nunes de Barros, Leonardo José Richtzenhain, Paulo Eduardo Brandão
Autor Correspondente: Sibele Pinheiro de Souza | [email protected]

Palavras-chave: Bovine coronavirus, restriction enzyme, genotypes, gene S.

Resumos Cadastrados

Resumo Inglês:

This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus
(BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was
concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation.