DEGRADATION OF H-ACID BY FREE AND IMMOBILIZED CELLS OF ALCALIGENES LATUS

Ano: 2010 | Volume: 41 | Número: 4

Autores: Usha M.S., Sanjay M.K., Gaddad S.M., Shivannavar C.T.

Palavras-chave: H-acid, biodegradation, immobilized cells, Alcaligenes latus

Resumo Inglês:

Alcaligenes latus, isolated from industrial effluent, was able to grow in mineral salts medium with 50 ppm
(0.15 mM) of H-acid as a sole source of carbon. Immobilization of Alcaligenes latus in Ca-alginate and
polyurethane foam resulted in cells embedded in the matrices. When free cells and immobilized cells were
used for biodegradation studies at concentration ranging from 100 ppm (0.3 mM) to 500 ppm (1.15 mM)
degradation rate was enhanced with immobilized cells. Cells immobilized in polyurethane foam showed
100% degradation up to 350 ppm (1.05 mM) and 57% degradation at 500 ppm (1.5 mM). Degradation rate
of Ca-alginate immobilized cells was less as compared to that of polyurethane foam immobilized cells.
With Ca-alginate immobilized cells 100% degradation was recorded up to 200 ppm (0.6 mM) of H-acid
and only 33% degradation was recorded at 500 ppm (1.5 mM) of H-acid. Spectral analysis of the products
after H-acid utilization showed that the spent medium did not contain any aromatic compounds indicating
H-acid degradation by A. latus.