CANDIDA ALBICANS: GENOTYPING METHODS AND CLADE RELATED PHENOTYPIC CHARACTERISTICS

Brazilian Journal Of Microbiology

Endereço:
Av. Prof. Lineu Prestes, 2415 Cidade Universitária
São Paulo / SP
Site: http://www.scielo.br/scielo.php?script=sci_serial&pid=1517-8382
Telefone: (11) 3813 9647
ISSN: 15178382
Editor Chefe: Adalberto Pessoa Junior
Início Publicação: 31/12/1970
Periodicidade: Trimestral
Área de Estudo: Microbiologia

CANDIDA ALBICANS: GENOTYPING METHODS AND CLADE RELATED PHENOTYPIC CHARACTERISTICS

Ano: 2010 | Volume: 41 | Número: 4
Autores: Juliana P. Lyon, Karen C.M. Moraes, Leonardo M. Moreira, Flávio Aimbire, Maria Aparecida de Resende
Autor Correspondente: Juliana P. Lyon | [email protected]

Palavras-chave: Candida albicans, clonal reproduction, microevolution, genotyping systems, phenotypic characteristics

Resumos Cadastrados

Resumo Inglês:

Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, and
DNA microsatellite analysis, have been employed to genotype Candida albicans. The genotype analysis
allows to group strains in clades, that is, a group composed of one ancestor and its descendants. These
genotype studies demonstrate that clades distribution is influenced by geographic area as well as that
antifungal resistance is associated with particular clades. These findings suggested that C. albicans
reproduces mainly in a clonal manner, with certain degree of DNA microevolution. Additionally, virulence
factors and site of isolation have also been associated with clade specificity. The present article is a brief
review about the methods used for Candida genotyping and the correlated clade systems established.
Special emphasis is given to Ca3 hybridization, MLST, and Microsatellites. The present work is also
focused on the phenotypic and physiological traits associated with Candida clades.