Biotechnological Potential of Alternative Carbon Sources for Production of Pectinases by Rhizopus microsporus var. rhizopodiformis

Brazilian Archives Of Biology And Technology

Endereço:
Rua Professor Algacyr Munhoz Mader 3775, CIC
Curitiba / PR
0
Site: http://www.scielo.br/babt
Telefone: (41) 3316-3012
ISSN: 15168913
Editor Chefe: Carlos Ricardo Soccol
Início Publicação: 30/11/1946
Periodicidade: Bimestral
Área de Estudo: Biologia geral

Biotechnological Potential of Alternative Carbon Sources for Production of Pectinases by Rhizopus microsporus var. rhizopodiformis

Ano: 2011 | Volume: 54 | Número: 1
Autores: André Ricardo de Lima Damásio, Alexandre Maller, Tony Márcio da Silva, João Atílio Jorge, Hector Francisco Terenzi, Maria de Lourdes Teixeira de Moraes
Autor Correspondente: André Ricardo de Lima Damásio | [email protected]

Palavras-chave: agro industrial residues, lemon peel, pectin lyase, polygalacturonase, r microsporus var, rhizopodiformis

Resumos Cadastrados

Resumo Inglês:

Fungi collected from Brazilian soil and decomposing plants were screened for pectinase production. R. microsporus
var. rhizopodiformis was the best producer and was selected to evaluate the pectic enzyme production under several
nutritional and environmental conditions. The pectinase production was studied at 40ºC, under 28 carbon sourcessupplemented
medium. The inducer effect of several agro-industrial residues such as sugar cane bagasse, wheat
flour and corncob on polygalacturonase (PG) activity was 4-, 3- and 2-fold higher than the control (pectin). In
glucose-medium, a constitutive pectin lyase (PL) activity was detected. The results demonstrated that R.
microsporus produced high levels of PG (57.7 U/mg) and PL (88.6 U/mg) in lemon peel-medium. PG had optimum
temperature at 65 ºC and was totally stable at 55 ºC for 90 min. Half-life at 70 ºC was 68 min. These results
suggested that the versatility of waste carbon sources utilization by R. microsporus, produce pectic enzymes, which
could be useful to reduce production costs and environmental impacts related to the waste disposal.